Oxytenanthera abyssinica rhizomes have been used in the treatment of aliments varying from colics, diabetes, polyuria and albuminiura, these ethanomedicinal potencies have been attributed to its rich phytochemical contents and free radical scavenging capacity. This work is aimed at investigating the effect of crude oil pollution on total phenolic, flavonoids and free radical scavenging potentials of methanol extract of Oxytenanthera abyssinica rhizomes. The ability of the plant extracts to scavenge 2,2- diphenyl- 1- picylhydrazyl (DPPH), hydroxyl radical (.OH), superoxide radical (O2-), nitric oxide radical (NO.) and ability to inhibit lipid peroxidation was assayed following standard methods, spectrophotometric method was used to assay for total phenolic and flavonoid content. Non crude oil polluted O. abyssinica extract (NCOPOAE) and crude oil polluted O. abyssinica extract(COPOAE) in a concentration dependent manner showed a potent antioxidant activity in DPPH radical scavenging assay (EC50= 1.03 ± 0.01µg/ml) and (EC50 = 1.04 ± 1.01µg/ml), inhibited hydroxyl radical (EC50 = 1.17 ± 1.02µg/ml) and (EC50 = 1.46 ± 1.21µg/ml), superoxide anion radical (EC50 =1.30 ± 1.21µg/ml) and (EC50 =1.51 ± 1.42µg/ml) and nitric oxide (EC50 = 1.40 ± 1.23µg/ml) and (EC50= 1.53 ± 1.42µg/ml) also inhibited non enzymatic lipid peroxidation (EC50 = 1.58 ± 1.42µg/ml) and (EC50 = 2.47 ± 2.13µg/ml) and had more of phenolic and flavonoid contents than COPOAE. This result indicates that NCOPOAE contains more antioxidant compounds than COPOAE, which could make it more potent in ethanomedicinal use.
Oxytenanthera abyssinica rhizomes, crude oil pollution, antioxidant, lipid peroxidation, reactive oxygen species
International Journal of Trend in Scientific Research and Development - IJTSRD having
online ISSN 2456-6470. IJTSRD is a leading Open Access, Peer-Reviewed International
Journal which provides rapid publication of your research articles and aims to promote
the theory and practice along with knowledge sharing between researchers, developers,
engineers, students, and practitioners working in and around the world in many areas
like Sciences, Technology, Innovation, Engineering, Agriculture, Management and
many more and it is recommended by all Universities, review articles and short communications
in all subjects. IJTSRD running an International Journal who are proving quality
publication of peer reviewed and refereed international journals from diverse fields
that emphasizes new research, development and their applications. IJTSRD provides
an online access to exchange your research work, technical notes & surveying results
among professionals throughout the world in e-journals. IJTSRD is a fastest growing
and dynamic professional organization. The aim of this organization is to provide
access not only to world class research resources, but through its professionals
aim to bring in a significant transformation in the real of open access journals
and online publishing.